Year of Establishment: June 1977

Dept of Microbioogy
FY Photo Journal:

This year we are starting with a new conept of photo/Video Journal along with regular laboratory books and journals

Almost every student now possesses a phone with good quality camera. We are going to exploit this by asking students to records their results, experimental procedures, whatever they think relevant and share it with the whole class /public. We hope that this will provide them with real time recall system and help remember exactly what they didd while performing experiments.

FYBSc students are uploading photos of all equipments on the group album with necessary description. Once the collection is complete, they will move to videos recording of instrument operations etc. -  Here is our first output 

Special Features of the Department:
  • Active participation in CPE, FIST, DBT sponsered Star College Program 
  • Departmental facilities are available to students through out the year including vacations. (Lab is closed only on Public holidays announced by College)
  • Extensive and regularly updated book bank with not only textbooks but also reference in all related subjects (more than 1500 books and 150 titles in specialized area)
  • Conducts courses for specialized needs of undergraduate students of Microbiology
  • Actively encourages students to undertake research projects - Project based / inquary based learning
  • Extensive collection of educational / subject related software (in public domain), freely available for copy and also download (only few due to size restrictions) from dept site
  • Dedicated computer facilities for active use of student: All computers are on LAN with Windows / Linux / Knowpix operating systems


e-Learning Classroom, All Labs, Labs, office with LAN /Intranet/Internet,

  "Moodle" based educational server system for online type of courses Bioinformatics, Molecular Biology
  Internet connection for unlimited internet connectivity for academic use - (regulated use)
   e-Portfolio / Personalized Learning Environment
  • Conducted 4 Refresher courses for UGC ASC, Univ. of Mumbai (1999, 2000, 2003, 2004)
  • Eco-biodiversity Net for Microbiology from 1999 as a intercollegiate activity
  • Undertakes commercial sample analysis, process development, consultation services for industry

Courses offered:

Aided Courses

Unaided Courses (Departmental)

Not affiliated to/recognized by any university or  Edu. Body

  B Sc Microbiology
  M Sc Microbiology (by Research Only)*
  Ph DMicrobiology*

Online Certificate Course in




Molecular Biology

Unaided Courses

M.Sc Microbiology by Papers from 2008-09

 * Department is recognized to register total 10 students together for M Sc by Research & Ph D in Microbiology

Faculty of the Department

Name, Qualifications

& Designation

Research Guide for

 Recognized University PG teacher (for M.Sc. theory)

Area of Interest for Research

Dr R P Phadke

M Sc, Ph D

Associate Professor,

Head of the Department

M Sc & Ph DMicrobiology

M Sc Microbiology &  M.Sc LifeScience

Lab on Chip/Paper, Systems & Computational Biology, Bioinformatics· Microarray data analysis Non-aqueous enzymology for biotransformations / process modeling / Flux analysis· Membrane less fuel cell· Bio-fertilizer development , Science Education

Mrs. L R Phadke

M Sc, M Phil

Associate Professor

M ScMicrobiology

 M Sc Microbiology

Molecular Biology, Applied Microbiology, Environmental Science, Biodiversity

Dr D Vora

M Sc, Ph D,

Associate Professor

M Sc & Ph DMicrobiology

 M Sc Microbiology &  M.Sc LifeScience

Regulation and Production of Enzymes of Bacillus Spp.· Germination and differentiation of spore bearers· Cultivation and physiology of Fresh water algae· Control of Microorganisms wrt cosmetics/ personal care products

Mrs. U N Palan

M Sc, M Phil

Associate Professor

M ScMicrobiology

 M Sc  Microbiology

Immunomodulation, Biodiversity, Medical microbiology Rapid Diagnostics

Mrs. A S Lokur

M Sc, M Phil

Associate Professor

M ScMicrobiology

 M Sc  Microbiology

Patent law' Biodiversity Environmental Microbiology Miniaturization in diagnostics

Ms V Shukla

M Sc,

Associate Professor

(on FIP) 


 M Sc  Microbiology

Studies in Spirulina, Extremophiles

Mr. V M Tupe

M Sc,

Assistant Professor 




Class Sites

Class site concept was introduced last yea rfor TY Class and now adapted for all classes. Such website is now being used for recording happenings in every class, collecting student submissions, being used as a backbone during regular classroom teaching as well as a medium for sharing ideas, posing and solving ex-tempo questions. We are still exploring possible uses. Here are links for few

FY Class Site          SY Class Site        TY Class Site     Microfluidic Project Site    M Sc Project Site

FY Micro Wiki Project


This page provides a brief background and link to this wiki project pages

This project is undertaken by teachers and students of FYBSc Microbiology 2009-10 batch and involves use of semantic wiki technology for enhancing the learning experience of Microbiology

Semantic wiki technology allows use of subject specific ontology to enhance basic wiki software. This allows collaborative learning as well as individualized learning to students. For further details of the software please visit media-wiki as well as semantic media wiki websites (We are using Referata semantic wiki host for this purpose)

Media-wiki website link

Semantic wiki Referata help link

How to Participate

At present this project is open (for active participation) to FYBSc students of Dept of Microbiology. Any one can view the pages though but cannot contribute.

Students of other classess  (SY /TY), if intrested, can initiate their own class project. Please contact Head, Dept of Microbiology for further details.

FYBSc Microbiology students should get themselves registered for this project. Registration process involves

  1. Obtaining an individualised login id & password from Department or by sending email to Head Dept of Microbiology
  2. Go through orientation material that will briefly acquaint you  to concepts of wiki, semantic wiki and ontology so that you understand how to enter, edit, format text in our wiki
  3. Get familiar with the overall plan of this project and then help its further development and refinement by your active participation
  4. To explore the power of semantic wiki think deeply about subject structure, different possible organizations and interrelationships amongst different topics
  5. You do not need password or log-in ID for just browsing the wiki site.

    Website Address

    Click on the link to proceed to our wiki

    website :

    Check this page periodically to check for any changes

FYBSc Microbiology Syllabus


Syllabus for the F.Y.B.Sc.

Program: B.Sc.

Course : Microbiology

                           (Credit Based Semester and Grading System with effect from the academic year 2011–2012)




F.Y.B.Sc. Microbiology Syllabus

Restructured for Credit Based and Grading System

To be implemented from the Academic year 2010-2011


Semester I

Course Code





2 Credits

(45 lectures )

Unit I

Introduction to Microbiology and prokaryotic cell structure and function.

1.1   Histrory and scope of Microbiology

1.2   Procaryotic cell membranes – plasma membranes and internal membrane systems

1.3   Cytoplasmic matrix – Inclusion bodies, ribosome.

1.4   Bacterial chromosome and plasmids – source of information

1.5   Cell Wall

1.6   Components external to cell wall – Capsule, Slime Layer, S Layer, Pili, Fimbriae, Flagella

1.7 Bacterial endospores and their formation.

15 Lectures

Unit II

Eukaryotic cell structure and function.

  2.1 Overview of  Eucaryotic cell structure and Function.

2.2  The cytoplasmic matrix, microfilaments, Intermediate  filaments and microtubules

2.3  Endoplasmic reticulum and Golgi apparatus

2.4  Lysosomes and Endocytosis

2.5 Eucaryotic ribosomes, Mitochondria and Chloroplasts

2.6  Nucleus, Nucleolus, Cell division, Mitosis and eiosis

2.7  External cell coverings

2.8  Comparision between Procaryotic and Eucaryotic cell

15 Lectures

Unit III

Microbial Nutrition, cultivation, isolation and preservation.

3.1  Nutritional requirements – Carbon, Oxygen, Hydrogen, Nitrogen, Phosphorus, Sulfur and growth factors.

3.2  Nutritional types of microorganisms

3.3  Nutrient uptake mechanisms

3.4  Types of Culture media with examples

3.5  Isolation of microorganisms and pure culture techniques.

3.6  Preservation of microorganisms

15 Lectures



Course Code





2 Credits

(45 lectures )

Unit I : Microscopy.

1.1   Concepts

1.2   Lenses & bending of light

1.3   The Light Microscope

a)    Bright Field Microscope

b)    Resolution of the Microscope

1.4   The Electron Microscope

a)     The Transmission Electron  Microscope

b)    The Scanning  Electron  Microscope

1.5   Newer Techniques in Microscopy

a)     Confocal Microscopy

b)    Scanning Tunncling  Microscope Eg. The Scanning Tunneling Microscope.

The Atomic Force  Microscope

15 Lectures

Unit II : Contrast Enhancement techniques.

2.1  The Dark Field Microscope

2.2  The Phase Contrast Microscope

2.3  The Differential Inerference Contrast Microscope

2.4  The Fluorescence Microscope

2.5  Staining of specimens

a)  Flaxation

b)  Dyes & simple staining

c)  Differential  staining

d)  Staining of specific structures

2.6  Specimen preparation in TEM Staining,Shadowing with metals, Freeze


15 Lectures

Unit III : Control of Microorganisms.

3.1  Definitions of  frequently used terms

3.2  Pattern / Rate of Microbial Death

3.3  Conditions influencing the effectivencess of

       Antimicrobial Agents

3.4  Physical methods of Microbial Control

a) Heat : Moist & Dry     b) Low temperature

c) Filtration                     d) High pressure

e) Desiccation                 F)  Osmotic pressure      g)  Radiations

3.5  Chemical methods of Microbial Control

a)  Phenolics    b)  Biguanides- Chlorohexidine

c)  Alcohols     d)  Halogens

e)  Heavy Metals   f)  Quaternary ammonium compounds

g)  Surface active agents  h)  Aldehydes

i)  Sterilising gases     j)  Peroxygens     k)  Chemotherapeutic agents

3.6  Evaluation of effectiveness of Antimicrobial agent.



15 Lectures


Course Code






2  Credits



Unit I : Safety in Laboratory

Assignment : Contribution of a Scientist in the field of Microbiology

Unit II : Procaryotic & Eucaryotic cell structures-

a)Monochrome Staining

b)Negative Staining

c)Staining of specific structures:Cell  wall, Capsule, Endospores,

    Metachromatic Granules, Lipids,Flagella, Spirocheates

d) Permanent slides of Algae & Protozoa

Unit III : Preparation of Culture Media

a)Liquid medium(Nutrient Broth)

b)Solid Media(Nutrient agar ,Sabourauds agar)

c)Preparation of slant ,butts & plates

         Inoculation techniques and Study of Growth

a)Inoculation of Liquid Medium

b)Inoculation of Solid Media(Slants, Butts and Plates)

c)Study of Colony Characteristics of Bacteria

d)Study of Motility (Hanging Drop Preparation) e)Use of  Differential, Selective and Enriched Media Superimposed Blood Agar, Determination of Optimum growth conditions

a) Temp.

b) Ph




Unit I : Care of Microscope

     Study of Compound Light /Phase Contrast/Binocular Microscope

     Study of Bright Field & Darkfield Microscopy

   Unit II : Differential Staining- Gram Staining

   Unit III : Physical Method of control of microorganisms:

a)   Heat: Autoclaving,Fractional   Sterilization,Dry heat

b)  Bacteria Proof Filteration(Demonstration of membrane filtration)

c)  Effect of UV rays

d)  Effect of Dessication

e)  Effeect of high osmotic pressure

        Chemical methods of control of microorganisms:

a)   Effect of Phenolics(Disc Method)

b)  Oligodynamic action

c)  Effect of Surfactant ( Tween 80)

d)  Dyes –Disc Method

e)  Chemotherapeutic agent



Semester II


Course Code





2 Credits

(45 lectures )

Unit I

Microbial growth.

1.1  Definition of growth, Mathematical Expression, Growth curve

1.2  Measurement of growth

a)    Direct microscopic count – Breed’s Petroff – Hausser counting chamber, Hacmocytometer.

b)    Viable count – Spread plate and Pour plate technique

c)    Measurements of cell consituents.

d)    Turbidity measurements – Nephelometer and spectrophotometer techniques

e)    Measurements of cell constituents

1.3 Synchronous growth, Continuous growth (Chemostat and Turbidostat)

1.4 Growth yeild

1.5 Influence of environmental factors on growth.

1.6 Microbial growth in natural environment.

1.7 Counting viable non-culturable organisms-Quorum sensing techniques.


15 Lectures

Unit II

     Study of Yeast, Molds, Algae and Protozoa.

2.1  Classification, Morphological characteristics, cultivation, reproduction and significance.

a)  Yeast and molds

b)  Algae

c)  Protozoa

15 Lectures

Unit III

     Study of viruses, Rickettsia, Chlamydia, Actinomycetes and   


3.1  Viruses : Classification of viruses, General characteristics, Structure of T4 phage, viral Cultivation, inclusion bodies and their significance, Life cycle of coliphages, lytic  cycle and lysogeny.

3.2  Rickettsia : General characteristics, Diseases and vectors

3.3  Chlamydia : General  characteristics

3.4  Actinomycetes L General properties, cell wall type, whole cell sugar patterns. Characteristics as per new classification and significance.

3.5  Archaea : Characteristics of major archaeal groups.

15 Lectures




Course Code





2 Credits

(45 lectures)

Unit I: Microorganisms in air and soil.

1.1  Air Microbiology

      a)  Type & significance  of organisms

      b)  Techniques  of studying Air Microflora

1.2  Microorgnisms in Terrestrial Environments

      a)  Soil as an enviroment

      b)  Soils, Plants & Nutrients

      c) Diversity of soil microorganisms

      d) Formation of different soils

1.3  Biogeochmical cycling

       a) Carbon cycle  b) Nitrogen cycle    c) Phosphorous cycle  d)  Sulfar  cycle  e)  Iron cycle      f)  Manganese cycle       g)  Metal toxicity.

1.4  The Physical cycling

        a)  The Microenvionment & Niche        b)   Biofilms & Microbial mats

        c)  Microorganisms & Populations

1.5  Examination of Microbial Populations

a)  Examination  Technique

b)  Examination of community Structure

c)  Microbial activity & turnover

d)  Recovery or addition of individual microbes.

15 Lectures

Unit II:  Microorganisms in water and waste water.

2.1  Marine & fresh water environments

a)  Water as a microbial habitat

b)  Nutrient cycling

c)  Fresh Water environments, Glaciers & permanently frozen lakes

            Streams  & rivers, Lakes

d) Marine environments, Estuaries & Salts marshes

The Photic zone,     The Benthic zone

e) Water purification & Sanitary analysis

2.2  Waste water Microbiology

a)  Measurement of waste water quality

b)  Waste water treatment.

15 Lectures

Unit III:  Microbial Interactions.

3.1  Types of Microbial Interactions Mutulism, Cooperation, Commensalisms, Predation Parasitism, Amensalism, Competition

       3.2 Human Microbe Interactions

a) Normal flora of the human body : Skin, Nose & Nasopharynx, Oropharynx, Respiratory tract, Eye, External ear, Mouth, Stomach, Small intestine, Large intestine, Genitourinary tract

          b) Relationship between microbiota & the   host

          c) Gnotobiotic animals

3.3 Microbial associations with vascular plants

          a) Phyllosphere                                          b) Rhizosphere & Rhizoplane

          c) Mycorrhizae

          d) Nitrogen fixation : Rhizobia, Actinorhizae, Stem Nodulating rhizobia

          e) Fungal & Bacterial endophytes

          f) Agrobacterium & other plant pathogens

15 Lectures

Course Code






2  Credits



Unit I : Measurement of Microbial Growth :


b)Opacity Tubes

c)Viable Count

d)Growth Curve of E. coli

Measurement of cell dimensions-Micrometry

Unit II : Cultivation of Yeast & Molds:

a)      Cultivation on Sabourauds agar

b)     Static & Shaker Cultures

c)     Fungal Wet mounts &

Study of Morphological Characteristics. Mucor, Rhizopus,Aspergillus, Penicillium,


   Unit III : Slide Culture technique ( Actinomycetes)






Unit I : Study of air Microflora and Determination of Sedimentation Rate

  Study of Soil Microflora (Bacteria, Yeast and Molds, Actinomycetes)

  Winogradsky Column-Demonstation of Sulphur Cycle

  Study of Azotobacter

  Study of Rhizobia

  Crowded Plate Technique

Unit II : Bacteriological Analysis of Water, Study of Raw Sewage


Unit III : Normal Flora of: a)Skin b)Intestine



References :

List of books for USMB – 101, 102, 201 & 202.


1.            Prescott ,Hurley.Klein-Microbiology, 5th & 6th edition, International edition 2002 & 2006, McGraw Hill.

2.            Kathleen Park Talaro & Arthur Talaro - Foundations in Microbiology International edition 2002| McGraw Hill.

3.            Michael T.Madigan & J.M.Martin,Brock ,Biology of Microorganisms 11th Ed. International edition 2006 Pearson Prentice Hall.

4.            A.J.Salle,Fundamental Principles of Bacteriology.

5.            Stanier.Ingraham et al ,General Microbiology 4th & 5th Ed. 1987, Macmillan Education Ltd.


Allocation of Time per Credit :- 1 Credit = 30 to 40 hours

Total contact Hrs :- 468 Hrs. per Semester i.e. 936 Hrs. per year.

Ratio of Instruction : Self Study :– (i) Theory – 1:1, (ii) Practicals – 4:1 


The time duration per credit is divided into two parts:


Approximately fifty percent of the time will be spent on class room instructions including practical as prescribed by the University.


Rest of the time spent as notional hours.-30-40 hrs/credit


Notional Hours: Module to be selected as per the department requirements.

  • Training for Assignment writing ,extra coaching for vernacular students, Journal writing
  • Student seminars or group discussion
  • Organize lectures or talks on the related subject.
  • Organize open day in the department with the participation of FY students for junior college students
  • Discuss career opportunities
  • Counselling Lecture
  • Industrial Visit, relevant to the subject
  • CD Shows/Film shows
  • E- book learning
  • Visit to an NGO, Science exhibition
  • Training   for participation in extra -curricular activities.
  • Interaction with parents.
  • Attending seminars, workshop,& conferences
  • Group activity/Self Study/Quiz.


Credit Assignment:  Semester I :



Learning Hours(h)

Lectures (L)






I   (USMB101)

45 L = 36 h




I   (USMBP101)


45 L = 36 h



II  (USMB102)

45 L = 36 h






45 L = 36 h



Total / Semester: 90 L = 72 h




Semester II:



Learning Hours (h)

Lectures (L)